Bilim
Pharmaceuticals in Kocaeli,
Turkey, recently performed a diffusion study that was published in Lab Asia Buyers Guide 2021/22
called, “Comparative
In Vitro Release Test Using Franz Diffusion Cell for Luliconazole 1% Cream With Reference Drug
Product.” They used Teledyne
Hanson’s Phoenix
DB-6 manual diffusion testing
system to gather the needed data. In vitro release testing (IVRT) was used to compare the Bilim product
Bilconazol 1% cream with Luzu Cream 1% as the reference drug. Several semi-solid formulations with different
concentrations were placed on the cellulose ester membranes. The receptor medium was a phosphate buffer
containing 50% ethanol. The DB-6 dry heat diffusion system was heated to 32 °C, with a stirring speed of 600
rpm. Samples were taken at 1 hour intervals for 6 hours. Two products were compared with each run. The six-cell
system was run in an ABABAB pattern with three cells used for each of the two products being
compared.
HPLC was used to analyze the samples. Results showed no statistically significant difference between the Bilim
product and the reference product. It was also determined that the temperature of the receptor environment was a
critical parameter for the study, and suggests the temperature of the lab should be monitored.
About Teledyne Hanson Diffusion systems.
The Teledyne
Hanson Phoenix dry heat diffusion systems make diffusion-cell testing faster, easier, and more
economical. The Phoenix DB-6 manual sampling system offers six-cell manual sampling in a compact footprint with an
advanced touch screen display. The Phoenix RDS Robotic Diffusion Station fully automates sampling, collection, and
media replace with the ability to run up to 24 cells at once. Both systems rely on the new Phoenix™ dry heat
diffusion cell, made from precision borosilicate glass, with mixer inserts engineered to produce instant
homogenization of receptor media.
